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. 2012 Jul 25;7(7):e41275. doi: 10.1371/journal.pone.0041275

Figure 2. Ng targets and relocates dendritic CaM closer to plasma membrane of the postsynaptic compartment.

Figure 2

(A) Representative images of immunogold-EM for CaM in control and Ng-overexpressing neurons. Scale bar, 100 nm. (B) Frequency histograms of CaM (n = 214) in control neurons and Ng-inf (n = 319) neurons. Normalized radial distance was measured the same way as described in Figure 1. Note the increased fraction of CaM at 0.1 interval in Ng-expressed condition. (C) Cumulative probabilities of normalized radial distance of CaM particles located within 0.2 of the normalized radial distance, where Ng was found to be concentrated in our previous study. Ng targeted CaM closer to the plasma membrane (p = 0.05). (D) Ng did not change the global distribution of CaM in the spine (PSD: postsynaptic density; E: extrasynaptic membrane; I: intraspine). The p-value for comparing cumulative distribution was calculated using Kolmogorov-Smirnov test. The p-value for comparing global CaM distribution in different sub-compartments was calculated using Chi-squared test.