. 2012 Jul 25;7(7):e41405. doi: 10.1371/journal.pone.0041405

Table 2. Comparisons between the lymphocyte subpopulation counts at different sampling sites.

Relative counts									Absolute counts
Lymphocyte subpopulation	Sites	ARD	Univariate	T/W	Mixed	Mixed joint	Bonferroni	Benjamini	ARD	Univariate	T/W	Bonferroni	Benjamini
CD4⁺CCR7⁺CD62L⁺CD45RA⁺	RE	−9.6	0.071	W	0.0092*	0.0011*	0.0012	0.0089	−9.4	0.003*	W	0.0012	0.0051*
CD4⁺CCR7⁺CD62L⁺CD45RA⁺	DE	−10.5	0.014*	W	0.0064*	0.0011*	0.0012	0.0089	−9.8	0.064	W	0.0012	0.0051
CD8⁺CCR7⁺CD62L⁺CD45RA⁺	DE	−4.3		T	0.0067*	0.0011*	0.0012	0.0089	−3.6	0.078	W	0.0012	0.0051
CD4⁺CCR7⁻CD62L⁺CD45RA⁺	DE	−2.3		T	0.075	0.0011*	0.0012	0.0089
CD4⁺CCR7⁻CD62L⁺CD45RA^-	DE	3.8	0.087	T	0.037*	0.0011*	0.0012	0.0089				0.0012	0.0051
CD8⁺CCR7⁻CD62L⁺CD45RA^-a	RE	9.6	0.047*	T	0.059	0.0011*	0.0012	0.0089
CD8⁺CCR7⁻CD62L⁺CD45RA^-	DE	8.4	0.0066*	T	0.0011*	0.0011*	0.0012	0.0089*	9.1	0.043*	T	0.0012	0.0051
CD4⁺CCR7⁻CD62L⁻CD45RA^-	RE	9.1	0.017*	T	0.0023*	0.0011*	0.0012	0.0089	10.0	0.036*	T	0.0012	0.0051
CD4⁺CCR7⁻CD62L⁻CD45RA^-	DE	9.9	0.000097*	T	0.0001*	0.0011*	0.0012*	0.0089*	10.4	0.00032*	T	0.0012*	0.0051*
CD8⁺CCR7⁻CD62L⁻CD45RA^-	RE	8.4	0.042*	T	0.035*	0.0011*	0.0012	0.0089
CD8⁺CCR7⁻CD62L⁻CD45RA^-	DE	5.5	0.037*	T	0.066	0.0011*	0.0012	0.0089
CD4⁺CD25⁺foxp3⁺	RD	−4.3	0.062	T			0.0012	0.0089
CD4⁺CD25⁺foxp3^+b	RE	−5.1	0.062	W	0.035*	0.0011*	0.0012	0.0089

Lymphocyte subpopulation counts are shown for all comparisons with P<.10 for both the relative and absolute cell counts. The univariate T/W method shows the P value for the two-sided paired t-test ‘T’ (‘Ln’ when a natural logarithm was applied) or Wilcoxon paired-matched signed rank (‘W’) in case normality didn’t hold. The Bonferroni and Benjamini columns show the adjusted significance levels according to the method applied. Sensitivity for outliers was performed for the relative cell counts. The mixed column shows all comparisons with P<.10 assessed as contrasts within a multivariate analysis for the relative counts for the lymphocyte subpopulations using SAS 9.2. The mixed joint column shows the multivariate P value. See Materials & Methods for more information.

Results annotated with ‘*’ are considered significant for the method applied. Sites comparison between sampling sites; ARD average relative difference; PLT platelets; NGC neutrophils; WBC white blood cells; RBC red blood cells; HCT hematocrit; RD radial artery vs. dorsal hand veins; RE radial artery vs. antecubital veins; DE dorsal hand veins vs. antecubital veins.

After omission of outliers the P value from the paired t-test was 0.093 with ARD 6.7%. The multivariate analysis showed a P value of 0.014.

After omission of outliers the P value from the paired t-test was 0.11 with ARD 3.3%. The multivariate analysis showed a P value of 0.042.