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Characterization of Amir_2000, Staur_3140, and related enzymes. A, SDS-PAGE of Ni-NTA purified cyclases. B, TLC analysis of the cylases' products with DAHP as substrate. C, TLC analysis of the cyclases' products with sedoheptulose 7-phosphate as substrate. D, Chemical structures of 2-epi-5-epi-valiolone, 5-epi-valiolone, 2-epi-valiolone, valiolone, and desmethyl-4-deoxygadusol.
