Table 2.

Sequence alignment of the proposed active site residues of currently known DHQS-like sugar phosphate cyclases

Protein	130	146	152	162	194	197	250	260	264	268	271	275	287	356
DHQS	R	D	K	N	E	K	K	E	R	N	H	H	H	K
aDHQS	R	D	K	N	E	R	K	D	R	N	H	H	H	K
DOIS	R	D	K	N	E	K	K	E	G	E	H	H	H	K
EEVS	R	D	K	N	E	K	M	E	R	D	H	P	H	P
EVS	R	D	K	N	E	K	K	E	R	N	H	H	H	R
DDGS	R	D	K	N	E	K	M	E	R	A	H	P	H	P

Multiple amino acid sequence alignment was conducted by ClustalW. Full-length amino acid sequences were aligned using the following parameters: protein weight matrix=Blosum, gap open penalty=15, gap extension penalty=0.2. Numbering is based on DHQS domain of AROM from A. nidulans. Proteins used for multiple sequence alignment are listed in Supplemental Table S1.