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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Mater. 2012 Jul 1;11(8):734–741. doi: 10.1038/nmat3361

Fig. 2. Tumour metastasis of 3D cultured B16-F1 cells in lung tissue of BALB/c mice.

Fig. 2

(a) B16-F1 tumour spheroids formed in soft 3D fibrin gel after 5-day culture were injected into tail vein of BALB/c mice with 10 cells. Mice were sacrificed after 2 months of injection and lung tissue image was recorded. B16-F1 cells cultured in 2D rigid dish were used as control. The results shown were a representative from three independent experiments. (b) Lung tissues of the above B16-F1 tumour spheroid treated BALB/c mice were analyzed by H&E staining. B16-F1 cells cultured on 2D rigid dish were used as control.