Table 2. 3D soft fibrin gels promote more efficient tumourigenicity.

B16-F1 cells were cultured on 2D soft fibrin gels (2D soft fibrin) (90-Pa) or in 3D soft fibrin gels (3D soft fibrin) (90-Pa) or on the rigid plastic (Plastic), all for 5 days. They were then subcutaneously injected into C57BL/6 mice with different cell numbers. CD133⁺CD44⁺ the B16-F1 cells were cultured for 5 days on rigid plastic and labeled with FITC-conjugated anti-CD133 and PE-conjugated anti-CD44 antibodies, and then sorted by FACS. The tumour formations were counted as a function of injected cell number.

Cell number	2D soft fibrin	3D soft fibrin	Plastic	CD133+ CD44+
100	0/6	2/6	0/6	0/6
1000	1/6	5/6	0/6	2/6
10000	5/6	6/6	3/6	4/6
100000	6/6	6/6	6/6	6/6