Figure 5.

Activation of caspase 9 by cluvenone in CEM cells. CEM cells were treated with 0.1% DMSO or 1 μM cluvenone for 5 h and cell protein was extracted. Caspase 9 activity was measured using a colorimetric assay in which cell lysates were incubated with caspase−9 substrate for 2 h at 37°C and absorbance was measured at 405 nm. All conditions were performed in triplicate and error bars represent SD.