Fig. 3.

Vector maps used with EPL (a and b) and PTS approaches (c and d). a P12 domains were cloned at the front of Mxe GyrA intein fused with CBD in pTWIN1 vector. b P3–5 domains were cloned after Ssp DnaB intein fused with CBD domain in pTWIN1 vector. c P12 domains were cloned at the front of N-intein in pMMRSF17 as a fusion protein with yeast Smt3 protein. d P3–5 domains were cloned after C-intein in pMMBAD16