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. 2012 Aug;26(8):3306–3320. doi: 10.1096/fj.12-207597

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Figure 6. — LPA mediated the tumor-promoting activity of ascites fractions. A) Effect of PLA₂ and ATX inhibitors on ascites S1-induced migration. B) Effect of heat treatment of ascites fractions or pretreatment of SKOV3 cells with Ki16425 (10 μM) on EOC cell migration. C) Effect of siRNA-mediated knockdown of LPA_1–4 in SKOV3 cells on S1-induced cell migration. D) Effect of LPI, oleic acid or lyso-PAF on SKOV3 cell migration. E) Normalized LPA receptor mRNA levels from siRNA-treated SKOV3 cells determined by quantitative real-time PCR. Data are means ± sd of 3 experiments. *P < 0.05, ***P < 0.001 vs. control (CON).