Table 4.
Lipid generation in ascites samples ex vivo
| ID, fraction, and condition | Total LPC | AA | Total LPA |
|---|---|---|---|
| A5 S1 | |||
| 0 h | 73.4 | 1.1 | 7.5 |
| 48 h | 49.3 | 3.2 | 43.2 |
| MAFP | 65.8 | 3.1 | 14.2 |
| BrP-LPA | 63.7 | 2.8 | 34.4 |
| A6 S1 | |||
| 0 h | 51.3 | 1.9 | 4.3 |
| 48 h | 49.1 | 6.7 | 34.9 |
| MAFP | 55.3 | 5.4 | 6.9 |
| BrP-LPA | 57.2 | 4.8 | 24.5 |
| A7 S1 | |||
| 0 h | 39.2 | 2.1 | 4.9 |
| 48 h | 28.2 | 5.4 | 42.5 |
| MAFP | 46.2 | 4.3 | 12.0 |
| BrP-LPA | 56.2 | 4.8 | 39.2 |
| A5 S4 | |||
| 0 h | 68.5 | 8.0 | |
| 48 h | 45.7 | 44.6 | |
| MAFP | 64.6 | 14.2 | |
| BrP-LPA | 64.3 | 33.5 | |
| A6 S4 | |||
| 0 h | 56.4 | 5.8 | |
| 48 h | 41.7 | 36.8 | |
| MAFP | 54.8 | 12.3 | |
| BrP-LPA | 58.6 | 29.1 | |
| A7 S4 | |||
| 0 h | 44.6 | 6.8 | |
| 48 h | 31.8 | 47.3 | |
| MAFP | 49.4 | 16.1 | |
| BrP-LPA | 58.3 | 45.1 |
S1 and S4 ascites fractions were incubated at 37°C for 48 h in the presence or absence of inhibitors (100 μM). Lipids were analyzed by LC-MS and are expressed as micromolar concentration in ascites.