Table 5.
Individual LPC and LPA lipid species in control and incubated ascites fractions from 3 patients with EOC
| ID, fraction, and condition | LPC |
LPA |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 16:0 | 18:2 | 18:1 | 18:0 | 20:4 | 22:6 | 16:0 | 18:2 | 18:1 | 18:0 | 20:4 | 22:6 | |
| A5 S1 | ||||||||||||
| Control | 43.9 | 7.05 | 8.96 | 9.80 | 2.34 | 0.77 | 2.10 | 1.77 | 0.90 | 0.30 | 2.04 | 0.31 |
| 37°C, 48 h | 31.7 | 2.37 | 4.51 | 8.88 | 1.03 | 0.48 | 18.1 | 6.72 | 6.25 | 2.60 | 7.62 | 1.59 |
| 37°C, 48 h + MAFP | 39.3 | 5.82 | 7.90 | 9.13 | 2.13 | 0.89 | 3.88 | 3.46 | 1.72 | 0.61 | 3.83 | 0.63 |
| 37°C, 48 h + BrP-LPA | 39.8 | 3.58 | 6.84 | 10.9 | 1.50 | 0.78 | 13.2 | 6.39 | 4.55 | 1.95 | 6.76 | 1.27 |
| A5 S4 | ||||||||||||
| Control | 41.4 | 6.04 | 8.37 | 9.30 | 2.21 | 0.66 | 2.20 | 1.99 | 0.94 | 0.34 | 2.15 | 0.34 |
| 37°C, 48 h | 28.9 | 2.52 | 4.55 | 7.96 | 1.05 | 0.44 | 17.5 | 7.49 | 6.68 | 2.71 | 8.13 | 1.69 |
| 37°C, 48 h + MAFP | 38.2 | 5.96 | 7.91 | 8.85 | 2.28 | 0.90 | 3.95 | 3.49 | 1.79 | 0.61 | 3.57 | 0.61 |
| 37°C, 48 h + BrP-LPA | 38.5 | 3.95 | 7.79 | 11.1 | 1.80 | 0.81 | 12.8 | 6.26 | 4.51 | 1.89 | 6.61 | 1.17 |
| A6 S1 | ||||||||||||
| Control | 33.0 | 2.37 | 5.40 | 6.96 | 2.67 | 0.58 | 1.13 | 0.50 | 0.39 | 0.20 | 1.80 | 0.22 |
| 37°C, 48 h | 32.7 | 1.44 | 3.99 | 9.00 | 1.28 | 0.39 | 14.9 | 2.85 | 4.61 | 2.23 | 8.76 | 1.33 |
| 37°C, 48 h + MAFP | 33.7 | 3.17 | 6.15 | 7.69 | 3.27 | 0.84 | 1.70 | 0.83 | 0.66 | 0.31 | 2.94 | 0.34 |
| 37°C, 48 h + BrP-LPA | 36.1 | 2.12 | 5.99 | 9.60 | 2.33 | 0.70 | 9.65 | 2.36 | 2.89 | 1.38 | 7.11 | 0.90 |
| A6 S4 | ||||||||||||
| Control | 35.8 | 2.98 | 5.92 | 7.47 | 3.13 | 0.66 | 1.72 | 0.62 | 0.59 | 0.26 | 2.32 | 0.26 |
| 37°C, 48 h | 26.9 | 1.62 | 3.62 | 7.27 | 1.61 | 0.46 | 14.3 | 3.34 | 4.49 | 2.21 | 10.70 | 1.44 |
| 37°C, 48 h + MAFP | 33.7 | 3.23 | 6.12 | 7.52 | 3.18 | 0.73 | ||||||
| 37°C, 48 h + BrP-LPA | 35.0 | 2.36 | 6.64 | 10.5 | 2.87 | 0.88 | 10.3 | 3.10 | 3.08 | 1.57 | 9.73 | 1.07 |
| A7 S1 | ||||||||||||
| Control | 20.8 | 4.45 | 5.36 | 4.95 | 2.88 | 0.48 | 0.70 | 1.07 | 0.48 | 0.20 | 2.21 | 0.25 |
| 37°C, 48 h | 16.9 | 1.51 | 2.34 | 6.03 | 1.04 | 0.23 | 16.2 | 4.98 | 6.17 | 2.85 | 10.54 | 1.49 |
| 37°C, 48 h + MAFP | 26.1 | 4.19 | 5.89 | 6.52 | 2.60 | 0.57 | 2.00 | 2.40 | 1.31 | 0.48 | 5.06 | 0.61 |
| 37°C, 48 h + BrP-LPA | 33.5 | 2.62 | 6.17 | 11.2 | 1.91 | 0.50 | 13.3 | 5.22 | 5.61 | 2.50 | 11.01 | 1.33 |
| A7 S4 | ||||||||||||
| Control | 24.0 | 4.50 | 6.11 | 6.26 | 3.04 | 0.48 | 1.25 | 1.38 | 0.72 | 0.29 | 2.82 | 0.30 |
| 37°C, 48 h | 18.7 | 1.78 | 2.61 | 7.10 | 1.13 | 0.25 | 17.3 | 5.84 | 7.25 | 3.01 | 11.82 | 1.65 |
| 37°C, 48 h + MAFP | 27.8 | 4.62 | 6.30 | 6.98 | 2.78 | 0.60 | 2.95 | 3.24 | 1.83 | 0.66 | 6.57 | 0.72 |
| 37°C, 48 h + BrP-LPA | 34.2 | 2.91 | 6.47 | 11.8 | 2.08 | 0.56 | 15.9 | 6.16 | 6.35 | 2.92 | 12.09 | 1.29 |
S1 and S4 ascites fractions were incubated at 37°C for 48 h, and lipids were analyzed by LC-MS. Lipids are expressed as micromolar concentration in ascites. Inhibitors were added for the 48 h incubation (100 μM).