Figure 1.

miR-31 and FIH-1 are reciprocally expressed in corneal and limbal epithelia. A) Northern blots of miR-31 and U6 expression in HCEKs and HLEKs. B) Western blots of FIH-1, keratin 3, and GAPDH in HCEKs and HLEKs. C) RNA samples prepared from laser-capture microdissected populations of resting adult limbal and corneal epithelia were subjected to real-time PCR. miR-31 levels were significantly higher in corneal epithelium. D–F) FIH-1 immunostaining of human (D) and mouse (E, F) limbal (E; Lim) and corneal (F; Cor) epithelia. PC, peripheral cornea.