FIH-1 inactivates Akt signaling. Immunoblotting of FIH-1, p-Akt (S473), p-GSK-3β, GSK-3β, and GS in FIH-1-transduced (A), shFIH-1- transduced (B), and antago-31-treated HCEKs (C). Numbers below panels represent the normalized expression signals of proteins.