Figure 5.

Akt/GSK-3β signaling is directly affected by FIH-1. A) HCEKs transduced with LZRS or LZRS-FIH-1-cds were treated with a myristolated Akt for 3 d and stained with PAS with and without amylase to digest glycogen. B) Glycogen density was quantified using computer-assisted image analysis. FIH-1-cds significantly decreased glycogen stores, whereas myristolated Akt or myristolated Akt in combination with FIH-1-cds rescued glycogen. C) HCEKs transduced with LZRS or LZRS-FIH-1-cds were treated with a GSK-3β inhibitor for 1 d and stained with PAS with and without amylase to digest glycogen. D) Glycogen density was quantified using computer-assisted image analysis. GSK-3β inhibitor significantly increased glycogen in HCEKs transduced with either LZRS or FIH-1-cds.