Figure 2.

Effects of HSD on tissue structure and substrate storage in the heart. A, B) Cardiac size of chow-fed (solid bars) and HSD-fed (open bars) rats was assessed by normalizing heart weight to body weight (A) or heart weight to tibial length (B). C) Cardiac cell size and extracellular fibrosis were analyzed on H&E- and Masson's trichrome (MT)-stained cardiac tissue paraffin sections, respectively. D) Intracardiac glycogen and triglyceride levels were compared by PAS and oil red O (ORO) staining, respectively. E, F) Intracardiac glycogen (E) and triglycerides (F) were further quantified by enzymatic methods. Data represent means ± se from 6 animals/group. **P < 0.01 vs. chow.