Figure 8.

Transient, marked overexpression by adenovirus-mediated gene transfer induces persistent signaling by TRH-R1. A) [³H]MeTRH binding. After 24 h, HEK-EM293 cells infected with 0, 1, 3, or 10 MOI of AdCMVmTRH-R1 were incubated with 2 nM [³H]MeTRH for 4 h at 4°C, washed with ice-cold HBSS, and solubilized. B) Effects of increasing levels of TRH-R1 expression on basal, acute, and persistent IP1 signaling. HEK-EM293 cells were infected with 0 (nonspecific), 1, 3, or 10 MOI of AdCMVmTRH-R1. After 24 h, basal, acute, and persistent IP1 production was determined as described in Materials and Methods. Figure represents data from 1 of 2 experiments. Data are means ± range of duplicate measurements.