Table 1.
Construct | ELP | C-terminal functionality | MW (kDa) | Rh (nm) 37 °C1 | Rh (nm) 42 °C1 |
---|---|---|---|---|---|
Arg5-ELPBC | (Val-Pro-Gly-Val-Gly)40-(Val-Pro-Gly-X-Gly)60 [X=Ala:Gly] | Arg5 | 40.6 | 5.5 ± 0.1 | 21.0 ± 1.3 |
ELPBC | (Val-Pro-Gly-Val-Gly)40-(Val-Pro-Gly-X-Gly)60 [X=Ala:Gly] | none | 39.8 | 5.8 ± 0.2 | 20.5 ± 0.6 |
Arg5-ELP | (Val-Pro-Gly-X-Gly)100 [X=Ala:Gly] | Arg5 | 39.2 | 5.4 ± 0.2 | 5.2 ± 0.1 |
ELP at 10 μM in PBS. Data represents the average of 3 replicates ± SEM.