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DCN neurons projecting to the IC express VGLUT2 but not VGLUT1. After an injection of Fluorogold (FG) into the IC, large DCN neurons, presumably fusiform and giant cells, show immunoreactivity for FG (brown). Their cell bodies are positive for VGLUT2 mRNA (dark blue in A), and negative for VGLUT1 mRNA (B) Note that numerous granule cells express VGLUT1 only. Bar: 40 μm.
