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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Mol Microbiol. 2012 Mar 5;84(1):166–180. doi: 10.1111/j.1365-2958.2012.08017.x

Fig. 5. RsAFP2 affects bud neck size and septin localization.

Fig. 5

A, A SEP7-GFP-tagged strain (JRB247) was imaged after treatment with 50 µg/ml RsAFP2 in YPD/PDB for 2 h, and compared to an untreated control. The top panel represents the overlay of DIC (Differential Interference Contrast) and fluorescence microscopy images. Abnormal septin localization is indicated by arrows, including diffuse septin localization at the neck (a), side-by-side necks (b), septin spots in the daughter cell (c), and otherwise abnormal localization (d). B, The bud neck width/mother cell diameter ratio was measured in cells treated with 50 µg/ml RsAFP2 and untreated (−) cells. Values are statistically significant (p=3.68 × 10−22).