Fig. 6. RsAFP2 impairs the yeast-to hyphae transition in C. albicans.
A, Overnight grown C. albicans CAI4 cultures in YPD (2×108 cells/ml) were diluted in YPD/10%FCS (8×104 cells/ml) and incubated with different concentrations of RsAFP2 or 10 µg/ml inhibitor 235236. Using solid phase cytometry, the percentage of living yeast cells displaying hyphal morphology were determined. Data are means of triplicate measurements of one representative experiment out of three. B, Percentage survival (as determined by counting colony forming units) of caspofungin-treated cells in the absence (open bars) or presence of 5 µg/ml RsAFP2 (sublethal dose; black bars), normalized to that of the mock treated cells. 5 µg/ml RsAFP2 alone resulted in > 80% survival. Data represent mean ± SEM. *p<0.05; **p<0.01; ***p<0.001.