Table 1.
Primers used in this study
| Primer name | Oligonucleotide sequence (5′ to 3′) |
|---|---|
| For semiquantitative RT-PCR | |
|
rrs-F |
CCAGCAGCCGCGGTAAT |
|
rrs-R |
TTTACGCCCAGTAATTCCGATT |
|
ramA-F |
ATTTGAATCAGCCGTTACGTATTG |
|
ramA-R |
TGCAGGTGCCACTTGGAAT |
| For quantitative RT-PCR | |
|
gmk-f |
TTGGCAGGGAGGCGTTT |
|
gmk-r |
GCGCGAAGTGCCGTAGTAAT |
|
gyrB-f |
TCTCCTCACAGACCAAAGATAAGCT |
|
gyrB-r |
CGCTCAGCAGTTCGTTCATC |
|
rrs-f |
CCAGCAGCCGCGGTAAT |
|
rrs-r |
TTTACGCCCAGTAATTCCGATT |
|
ramA-f |
GCGTGAACGGAAGCTAAAAC |
|
ramA-r |
GGCCATGCTTTTCTTTACGA |
|
acrB-f |
TCGTGTTCCTGGTGATGTACCT |
|
acrB-r |
AACCGCAATAGTCGGAATCAA |
|
mdtE-f |
AGTCGCTGGATACCACCATC |
|
mdtE-r |
GATATTACGCACGCCGATTT |
|
tolC-f |
GCCCGTGCGCAATATGAT |
|
tolC-r |
CCGCGTTATCCAGGTTGTTG |
|
flhC-f |
ATATCCAGTTGGCGATGGAG |
|
flhC-r |
TTGCTCCCAGGTCATAAACC |
|
hilA-f |
CATGGCTGGTCAGTTGGAG |
|
hilA-r |
CGTAATTCATCGCCTAAACG |
|
invF-f |
TGAAAGCCGACACAATGAAAAT |
|
invF-r |
GCCTGCTCGCAAAAAAGC |
|
invA-f |
GGCGCCAAGAGAAAAAGATG |
|
invA-r |
CAAATATAACGCGCCATTGCT |
|
sipA-f |
TTTGGCTGTACGTTAGATCCGTTA |
| sipA-r | CCGCCGCTTTGTCAACA |