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. Author manuscript; available in PMC: 2013 Jul 6.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Jun 10;423(3):577–582. doi: 10.1016/j.bbrc.2012.06.008

Fig. 2.

Fig. 2

Trypsin digested BspLRR1 retains the ability to activate TLR2. HEK293 (hTLR2/hTLR1) (A) or THP-Blue (B) reporter cells with NF-κB inducible alkaline phosphatase were incubated with Pam3Cys (0.1 µg/ml), Ec LPS (1 µg/ml) or rBspLRR1 (5 µg/ml), rBspLRR1 trypsin digest (5 µg/ml), TdLrrA (5 µg/ml) rYopM (5 µg/ml) and rGST (5 µg/ml). For THP-Blue cells, pretreatment with either anti-TLR2 or isotype control antibody prior to stimulation was also carried out. The secreted alkaline phosphatase activity (NF-κB induction) in the medium was measured after 16–18 h. The data represent averages ± standard deviations for triplicate determinations from one three independent experiments yeilding similar results; *, p < 0.05.