Fig. 3.

Peptides derived from Lrr repeats 3–6 activate TLR2. (A) HEK293 (hTLR2/hTLR1) reporter cells were stimulated with synthetic peptides (50 µg/ml each) for 16 h. (B & C) The active peptides inducing TLR2 were further tested in reporter cell lines HEK293 (HTLR2/hTLR1) and THP-Blue in the absence or presence of TLR2 blocking (or isotype control) antibody and NF-κB induction was assessed by measuring secreted alkaline phosphatase as in Fig. 2. The data represent averages ± standard deviations for triplicate determinations from one two independent experiments yielding similar results; asterisks (p < 0.05) in panel A indicate significant activation of NF-κB activation by agonist treatment compared to non treatment, and in panel B they indicate significant blocking of activation by anti-TLR2 antibody compared to isotype control (B).