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. 2012 Jul 2;209(7):1379–1389. doi: 10.1084/jem.20112253

Figure 5.

Figure 5.

UNG sequence preference for error-free repair is conserved in B cells. Primary spleen B cells from UNG+/+ (n = 4), UNG−/− (n = 4), and AID−/− (n = 2) mice were isolated, stained with CFSE, and cultured in the presence of LPS, IL-4, and BAFF for 96 h. Cells that had undergone five or more divisions were sorted and Sµ region was PCR amplified and sequenced by NGS. (A) Mutation frequency in UNG+/+, UNG−/−, or AID−/− was calculated as in Fig. 2 B. Standard deviations are shown. Frequency values are shown in Table 3. (B) Absolute frequency of G/C transversions, G/C transitions, and A/T mutations calculated as in Fig. 3 D. (C) G/C transition frequencies in UNG+/+ and UNG−/− mice in individual WRCY and RGYW hotspots in Sµ sequence, normalized to the mean G/C transition frequency in each mouse. Standard deviations are shown. Shadowed hotspots (AACC and TACC) are not present in the Sμ sequence. **, P < 0.01; ***, P < 0.001. Reference Sμ sequence is shown in Fig. S1 B. Complete mutation data are shown in Table S2.