Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
mOrangeSTOP sequencing by Sanger and NGS
| Sequencing method | Number of positionsa | Number of reads (mean)b | Mutation numberc | Frequency (×10−4)d |
| Sanger | 222 | 101 | 31 | 13.8 |
| NGS | 222 | 87,009 | 22,370 | 11.1 |
Number of total cytosine in mOrange sequence.
Mean number of reads per cytosine.
Total mutation number in cytosines.
Mutation frequency calculated as c/(b × a).
