Figure 7.

Anti-Psl mAb Cam-003 is active in P. aeruginosa keratitis and thermal injury models. C3H/HeN mice were treated with control R347 antibody or Cam-003 at 45 mg/kg (A and B) or 15 mg/kg (C and D) 24 h before infection with 6077 (O11-cytotoxic, 2e6 CFU). The corneal infection model was performed as previously described (DiGiandomenico et al., 2007). Differences in pathology scores and viable CFU were determined by the Mann-Whitney U test. P = 0.0001 (A); P < 0.0001 (B); P = 0.0003 (C); P = 0.0015 (D). (E) The thermal injury model was performed using CD-1 mice as previously described (DiGiandomenico et al., 2007). Survival analysis from Cam-003– and R347-treated CF-1 mice in a P. aeruginosa thermal injury model after 6077 infection (2 × 10⁵ CFU; log-rank: R347 vs. Cam-003 15 mg/kg, P = 0.0094; R347 vs. Cam-003 5 mg/kg, P = 0.0017). (A–D) 12 animals were used in each group. (E) Number of animals in each treatment group (R347, n = 7; Cam-003 5 mg/kg, n = 8; Cam-003 15 mg/kg, n = 8). Results are representative data from five (A–D) and from three independent experiments (E).