Table 1.

Epitope mapping and identification of the relative affinity for each Psl mAb

Epitope	mAb	Kd	Phage library	Cell attachment max. inhibition	OPK EC50
		nM		µg/ml	µg/ml
Class 1	Cam-003	144.00	Naive	1.0	0.0220
	Cam-004	2,100.00	Naive	>30.0	0.2771
	Cam-005	8,400.00	Naive	>30.0	>30.0
Class 2	WapR-001	0.84	Patient	30.0	0.3100
	WapR-003	12.20	Patient	30.0	0.2778
	WapR-002	12.60	Patient	30.0	0.3960
Class 3	WapR-016	75.00	Patient	10.0	0.2417

Epitope mapping was performed by competition ELISA and confirmed using an Octet flow system with Psl derived from the supernatant of an overnight culture of PAO1. The results indicate that Psl antibodies bind to at least three epitopes, which we refer to as class I, II, and III. Class I and II antibodies do not compete for binding; however, the class III antibody, WapR-016, partially inhibits binding of the class I and II antibodies. Antibody affinity was determined by Octet binding assays using Psl derived from the supernatant of overnight PAO1 cultures. Antibody K_D was determined by averaging the binding kinetics of seven concentrations for each antibody. Also shown is a summary of cell attachment and OPK data experiments for each antibody. ELISA and antibody affinity was determined using three independent studies.