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Distribution of peripherin and NFL along the sciatic nerve. Triton X-100-insoluble fractions from consecutive 3 mm segments of the sciatic nerve were separated by SDS-PAGE, transferred to nitrocellulose membranes, and then probed by anti-peripherin and anti-NFL antibodies (A). Analyses of these immunoblots demonstrate that peripherin and NFL show the same proximal-to-distal distribution (B) and maintain a constant ratio along the sciatic nerve (C). Per, Peripherin.
