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rpoB gene SNP discrimination by the TB ID/R test. Synthetic 128-bp templates were amplified using bpHDA as described above and were hybridized to the chip, and CCD camera images were captured. (A) Representative images of mutations that affect each probe. NTC, no-template control; WT, wild-type M. tuberculosis. Capitalized bases indicate mutant positions. (B) Array map. HC, hybridization control; DC, detect control; F+DC, fiducial marker mixed with the detect control.
