Fig 4.

Performance of the TB ID/R assay for detecting synthetic mutants with mutations in the rpoB locus. Synthetic templates with mutations (indicated by capital letters) were amplified, tested by the TB ID/R assay, and subjected to CCD imaging, and images were analyzed as described above. Signals for each probe on each chip were plotted. Filled diamonds, probe WT1.3; open squares, probe WT2.2; filled triangles, probe WT3.4; open triangles, probe WT4.6; filled squares, probe WT5.7; open circles, probe WT7.5. Signals below 10 pixels are difficult to detect by the unaided eye.