Fig 2.

Comparison of the susceptibility of clinical strains of Leishmania (Viannia) to meglumine antimoniate and miltefosine evaluated as the percentage of cells infected and parasite burden. Using thresholds of susceptibility defined by ROC curve analyses, low susceptibility (tolerance/resistance) was overestimated in clinical strains by readout as the percentage of cells infected cells compared to readout as the parasite burden at discriminatory concentrations of meglumine antimoniate (n = 130) (A) and miltefosine (n = 125) (B). Discontinuous lines correspond to cutoff thresholds. Empty circles represent strains erroneously classified as having low drug susceptibility by readout as the percentage of cells infected.