Table 1.

Culture, PCR, and HRMA results for spontaneous bacterial peritonitis with Gram-typing probe analysis for polymicrobial samples

Sample	Culture result	16S PCR result	HRMA identification	Discordant analysisf
1	Staphylococcus aureus	Positive	Staphylococcus aureus	NA
2	MRSA	Positive	Staphylococcus aureus	NA
3	Staphylococcus sp.e	Positive	Staphylococcus hominis	NA
4	Enterococcus faecalis	Positive	Enterococcus faecalis	NA
5	Pseudomonas aeruginosa	Positive	Pseudomonas aeruginosa	NA
6	Coagulase-negative Staphylococcus sp.e	Positive	Staphylococcus saprophyticus	NA
7	Coagulase-negative Staphylococcus sp.e	Positive	Staphylococcus lugdunensis	NA
8	Enterococcus faecium	Positive	Enterococcus faecium	NA
9	Beta-hemolytic Streptococcus sp.e	Positive	Streptococcus agalactiae	NA
10	Coagulase-negative Staphylococcus sp.e	Positive	Staphylococcus saprophyticus	NA
11	Serratia marcescens	Positive	Serratia marcescens	NA
12	Enterococcus faecalis	Positive	Enterococcus faecalis	NA
13	Klebsiella sp. and Enterococcus sp.	Positive	Polymicrobiala	Positive by GPc and GNd
14	Enterococcus sp.	Positive	Unidentifiedb	Positive by GP
15	Enterobacter aerogenes and Escherichia coli and alpha-hemolytic Streptococcus sp.	Positive	Polymicrobiala	Positive by GP and GN
16	Streptococcus anginosus and Staphylococcus intermedius and Streptococcus constellatus	Positive	Polymicrobiala	Positive by GP
17	Escherichia coli and Streptococcus viridans	Positive	Polymicrobiala	Positive by GP and GN
18	Negative	Positive	Polymicrobiala	Positive by GP and GN
19	Negative	Positive	Polymicrobiala	Positive by GP and GN
20	Negative	Positive	Polymicrobiala	Positive by GN
21	Negative	Positive	Micrococcus luteus	NA

^aMultiple melting curve profiles were generated, indicating a polymicrobial infection.

^bA single melting curve profile was generated, but it was inconsistent with all organisms in the HRMA library.

^cGram-positive typing probe.

^dGram-negative typing probe.

^eFurther characterization was not possible due to lack of sample for sequencing analysis.

^fNA, not applicable.