Table 1.
List of primers used in this study
| Gene or fragment | Primer |
Amplicon (bp) | Reference | |
|---|---|---|---|---|
| Name | Sequence (3′-5′) | |||
| Gene | ||||
| tpi | tpi-F | AAAGAAGCTACTAAGGGTACAAA | 230 | 13 |
| tpi-R | CATAATATTGGGTCTATTCCTAC | |||
| tcdB | tcdB-F | GGAAAAGAGAATGGTTTTATTAA | 160 | 13 |
| tcdB-R | ATCTTTAGTTATAACTTTGACATCTTT | |||
| tcdA | tcdA-F | AGATTCCTATATTTACATGACAATAT | 369 (A+B+)/110 (A−B+)a | 13 |
| tcdA-R | GTATCAGGCATAAAGTAATATACTTT | |||
| tcdC | Tim2- | GCACCTCATCACCATCTTCAA | 345 | 28 |
| Struppi2 | TGAAGACCATGAGGAGGTCAT | |||
| cdtB | cdtBpos | CTTAATGCAAGTAAATACTGAG | 500 | 31 |
| cdtBrev | AACGGATCTCTTGCTTCAGTC | |||
| tetM | TETMd | TGGAATTGATTTATCAACGG | 1,000 | 27 |
| TETMr | TTCCAACCATACAATCCTTG | |||
| tetW | WRC1 | CATCTCTGTGATTTTCAGCTTTTCTCTCCC | 457 | 27 |
| WRC2 | AGTCTGTTCGGGATAAGCTCTCCGCCG | |||
| ermB | E5 | CTCAAAACTTTTTAACGAGTG | 711 | 29 |
| E6 | CCTCCCGTTAAATAATAGATA | |||
| Fragment | ||||
| B1 | B1C | AGAAAATTTTATGAGTTTAGTTAATAGAAA | 21 | |
| B2N | CAGATA ATGTAGGAAGTA AGTCTATAG | |||
| A3 | A3C | TATTGATAGCACCTGATTT ATATACAAG | 21 | |
| A4N | TTATCAAACATATATTTAGCCATATATC | |||
a
The amplicon size for toxin A-positive (A+) strains was 369 bp. However, toxin A-negative (A−) strains showed a smaller size, 110 bp.