Table 3.
Results of quantitative PCR measurements of marker organisms in vaginal samples identified as positive or negative for BV
| Organism | BV statusa | PCR resultb (no.) |
qPCR value (log10 copies/ml) |
|||
|---|---|---|---|---|---|---|
| Negative | Positive | 25th percentile | 50th percentile | 75th percentile | ||
| A. vaginae | Negative | 29 | 32 | <3.00 | 3.11 | 4.98 |
| Positive | 2 | 106 | 6.52 | 6.90 | 7.32 | |
| BVAB-2 | Negative | 50 | 11 | <3.00 | <3.00 | <3.00 |
| Positive | 11 | 97 | 5.38 | 6.04 | 6.64 | |
| G. vaginalis | Negative | 24 | 37 | <3.00 | 3.48 | 5.31 |
| Positive | 2 | 106 | 6.61 | 6.99 | 7.53 | |
| Megasphaera-1 | Negative | 51 | 10 | <3.00 | <3.00 | <3.00 |
| Positive | 14 | 94 | 6.18 | 6.89 | 7.18 | |
| L. crispatus | Negative | 35 | 26 | <3.00 | <3.00 | 7.55 |
| Positive | 97 | 11 | <3.00 | <3.00 | <3.00 | |
a
BV status was defined based on a combination of NS and Amsel criteria. BV-positive samples were those with NS values of 7 to 10 and samples with NS values of 4 to 6 that met Amsel criteria.
b
Samples that generated qPCR values below the lower limit of quantitation of the respective assays (1,000 copies/ml) were considered negative.