Table 4.
Results of logistic regression analysis of qPCR results for marker organism combinations
| No. of markers | Organism | BV statusa | PCR categoryb statistical metric value |
|||
|---|---|---|---|---|---|---|
| No. negative | No. positive | Sensitivity (%) | Specificity (%) | |||
| 2 | A. vaginae/BVAB-2 | Negative | 50 | 11 | 95.4 | 82.0 |
| Positive | 5 | 103 | ||||
| A. vaginae/G. vaginalis | Negative | 47 | 14 | 95.4 | 77.0 | |
| Positive | 5 | 103 | ||||
| A. vaginae/Megasphaera-1 | Negative | 53 | 8 | 94.4 | 86.9 | |
| Positive | 6 | 102 | ||||
| BVAB-2/G. vaginalis | Negative | 50 | 11 | 96.3 | 82.0 | |
| Positive | 4 | 104 | ||||
| BVAB-2/Megasphaera-1 | Negative | 54 | 7 | 92.7 | 88.5 | |
| Positive | 7 | 101 | ||||
| G. vaginalis/Megasphaera-1 | Positive | 53 | 8 | 93.5 | 86.9 | |
| Negative | 7 | 101 | ||||
| 3 | A. vaginae/BVAB-2/G. vaginalis | Negative | 51 | 10 | 95.4 | 83.6 |
| Positive | 5 | 103 | ||||
| A. vaginae /BVAB-2/Megasphaera-1 | Negative | 55 | 6 | 95.4 | 90.2 | |
| Positive | 5 | 103 | ||||
| A. vaginae/G. vaginalis/Megasphaera-1 | Negative | 53 | 8 | 94.4 | 86.9 | |
| Positive | 6 | 102 | ||||
| BVAB-2/G. vaginalis/Megasphaera-1 | Negative | 55 | 6 | 93.5 | 90.0 | |
| Positive | 7 | 101 | ||||
| 4 | A. vaginae/BVAB-2/G. vaginalis/Megasphaera-1 | Negative | 52 | 9 | 95.4 | 85.2 |
| Positive | 5 | 103 | ||||
a
BV status was defined based on a combination of NS and Amsel criteria. BV-positive samples were those with NS values of 7 to 10 and samples with NS values of 4 to 6 that met Amsel criteria.
b
Categorized using logistic regression analysis, with BV status as the dependent variable (negative = 0; positive = 1) and qPCR results from the 169 development phase samples for the different marker combinations as the independent variable.