Table 4.
HPV IU/GE detection per 5 μl in both single and multiple infectionsa
| HPV type | No. of HPV IU/GE | No. of data sets detecting HPV by the following assay/total no. of data sets using the assay (%): |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Commercial assays |
In-house assaysa |
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| Linear array (Roche) | InnoLiPA (Innogenetics) | CLART HPV 2/3 (Genomica) | Digene HPV genotyping LQ | Papillocheck microarray (Greiner Bio-One) | PCR Luminex (Multimetrix) | Digene HPV genotyping RH (Qiagen) | Hybribio microarray | DEIA line probe (Lab.Bio) | EASYChip (King Car) | LCD array (Chiron) | Other commercialb | All assays | Line blot | PCR-RFLP | Luminex | PGMY-CHUV | Type-specific PCR | Real-time PCR | PCR sequencing | Dot blot | Other in-housec | ||
| 16 | 5 | 3/17 | 10/12 | 8/8 | 2/3 | 4/4 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 7/9 | 85/127 (67) | 3/10 | 2/7 | 7/7 | 4/6 | 4/6 | 4/5 | 3/5 | 1/2 | 3/3 | |
| 16 | 50 | 16/17 | 12/12 | 3/3 | 7/9 | 118/127 (93) | 9/10 | 5/7 | 5/6 | 5/5 | 4/6 | 2/2 | |||||||||||
| 16 | 500 | 17/17 | 1/2 | 9/9 | 125/127 (98) | 10/10 | 7/7 | 6/6 | 5/6 | 5/6 | |||||||||||||
| 18 | 5 | 3/17 | 9/12 | 5/8 | 3/3 | NTc | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 6/9 | 72/119 (60) | 2/10 | 1/7 | 5/7 | 4/6 | 3/6 | 3/5 | 3/5 | 2/2 | 2/3 | |
| 18 | 50 | 17/17 | 12/12 | 5/8 | 6/9 | 106/119 (89) | 7/10 | 3/7 | 7/7 | 5/6 | 4/6 | 4/5 | 4/6 | ||||||||||
| 18 | 500 | 8/8 | 1/2 | 9/9 | 108/119 (91) | 8/10 | 6/6 | 5/6 | 5/5 | ||||||||||||||
| 6 | 50 | 4/17 | 11/12 | 6/8 | 4/4 | 2/3 | 1/2 | 2/2 | 2/2 | 2/2 | 8/8 | 64/110 (58) | 2/10 | 3/7 | 2/6 | 5/6 | 4/5 | 2/4 | 2/6 | 1/3 | |||
| 6 | 500 | 17/17 | 12/12 | 8/8 | NT | 3/3 | NT | 2/2 | 97/110 (88) | 8/10 | 6/7 | 4/6 | 6/6 | 4/5 | 3/4 | 3/6 | 1/2 | 3/3 | |||||
| 11 | 50 | 4/17 | 12/12 | 8/8 | 4/4 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 6/8 | 79/109 (72) | 6/10 | 4/7 | 6/6 | 5/6 | 4/5 | 2/4 | 1/6 | 2/2 | 3/3 | ||
| 11 | 500 | 16/17 | NT | NT | 8/8 | 100/109 (92) | 8/10 | 7/7 | 6/6 | 4/5 | 3/4 | 2/6 | |||||||||||
| 31 | 50 | 4/17 | 12/12 | 8/8 | 1/3 | 1/4 | 2/2 | 2/2 | 2/2 | 4/9 | 52/118 (44) | 3/10 | 3/7 | 3/6 | 2/6 | 4/5 | 2/3 | ||||||
| 31 | 500 | 15/17 | 3/3 | 4/4 | 1/2 | 84/118 (71) | 5/10 | 3/7 | 5/7 | 5/6 | 4/6 | 1/6 | 1/2 | 3/3 | |||||||||
| 33 | 50 | 4/17 | 10/12 | 8/8 | 3/3 | 4/4 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 7/8 | 76/117 (65) | 4/10 | 2/7 | 4/7 | 5/6 | 3/6 | 5/5 | 2/2 | 3/3 | |
| 33 | 500 | 16/17 | 8/8 | 97/117 (83) | 7/10 | 3/7 | 5/7 | 4/6 | 2/6 | ||||||||||||||
| 35 | 50 | 4/17 | 12/12 | 8/8 | 3/3 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 6/9 | 70/118 (59) | 5/10 | 1/7 | 5/7 | 5/6 | 3/6 | 2/5 | 1/6 | 1/2 | 3/3 | ||
| 35 | 500 | 14/17 | 4/4 | 9/9 | 98/118 (83) | 8/10 | 2/7 | 7/7 | 4/6 | 4/5 | 3/6 | 2/2 | |||||||||||
| 39 | 50 | 4/17 | 9/12 | 4/4 | 3/3 | 1/2 | 2/2 | 2/2 | 2/2 | 5/9 | 45/118 (38) | 3/10 | 3/7 | 3/6 | 1/6 | 4/5 | 1/2 | 2/3 | |||||
| 39 | 500 | 15/17 | 11/12 | 1/8 | 7/9 | 77/118 (65) | 6/10 | 5/7 | 6/6 | 3/6 | 5/5 | 3/3 | |||||||||||
| 45 | 50 | 4/17 | 7/12 | 1/8 | 3/3 | 3/4 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 9/9 | 71/118 (60) | 7/10 | 2/7 | 6/7 | 5/6 | 3/6 | 5/5 | 2/6 | 2/2 | 2/3 | |
| 45 | 500 | 17/17 | 12/12 | 4/8 | 4/4 | 1/2 | 98/118 (83) | 9/10 | 2/7 | 7/7 | 3/6 | ||||||||||||
| 51 | 50 | 4/17 | 11/12 | 8/8 | 1/3 | 4/4 | 3/3 | 1/2 | 2/2 | 2/2 | 2/2 | 8/9 | 69/117 (59) | 3/9 | 1/7 | 5/7 | 5/6 | 3/6 | 4/5 | 2/3 | |||
| 51 | 500 | 15/17 | 12/12 | 3/3 | 2/2 | 92/117 (79) | 5/9 | 2/7 | 6/7 | 6/6 | 4/6 | 5/5 | 1/2 | 3/3 | |||||||||
| 52 | 50 | 4/17 | 11/12 | 8/8 | 4/4 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 9/9 | 79/117 (67) | 4/9 | 3/7 | 7/7 | 5/6 | 3/6 | 5/5 | 1/6 | 1/2 | 3/3 | ||
| 52 | 500 | 17/17 | 12/12 | 1/3 | 2/2 | 98/117 (84) | 7/9 | 3/7 | |||||||||||||||
| 56 | 50 | 4/17 | 10/12 | 3/3 | 4/4 | 3/3 | 2/2 | 2/2 | 2/2 | 7/9 | 63/118 (53) | 5/10 | 1/7 | 6/7 | 3/6 | 3/6 | 4/5 | 2/2 | 3/3 | ||||
| 56 | 500 | 15/17 | 12/12 | 3/8 | 86/118 (73) | 8/10 | 7/7 | 4/6 | |||||||||||||||
| 58 | 50 | 4/17 | 1/12 | 8/8 | 1/3 | 3/4 | 1/3 | 2/2 | 2/2 | 2/2 | 2/2 | 7/9 | 62/118 (52) | 4/10 | 4/7 | 6/7 | 5/6 | 3/6 | 3/5 | 1/2 | 2/3 | ||
| 58 | 500 | 17/17 | 5/12 | 3/3 | 2/3 | 1/2 | 9/9 | 91/118 (77) | 7/10 | 5/7 | 7/7 | 4/5 | 3/3 | ||||||||||
| 59 | 50 | 3/17 | 1/12 | 7/8 | 1/3 | 4/4 | 3/3 | 1/2 | 2/2 | 2/2 | 2/2 | 7/9 | 52/117 (44) | 3/9 | 1/7 | 5/7 | 4/6 | 2/6 | 1/5 | 1/2 | 1/3 | ||
| 59 | 500 | 16/17 | 2/12 | 2/3 | 2/2 | 2/2 | 8/9 | 80/117 (68) | 6/9 | 2/7 | 6/7 | 5/6 | 3/6 | 3/5 | 2/3 | ||||||||
| 66 | 50 | 4/17 | 11/12 | 8/8 | 3/3 | 3/4 | 3/3 | 2/2 | 2/2 | 2/2 | 2/2 | 6/7 | 75/113 (66) | 4/9 | 3/7 | 6/7 | 5/6 | 3/5 | 3/6 | 1/2 | 3/3 | ||
| 66 | 500 | 14/17 | 4/4 | 2/2 | 7/7 | 95/113 (84) | 7/9 | 6/7 | 7/7 | 3/5 | 4/6 | ||||||||||||
| 68a | 50 | 1/12 | 2/2 | 2/6 | 10/61 (16) | 1/5 | 3/6 | 1/1 | |||||||||||||||
| 68a | 500 | nt | 4/12 | NT | NT | NT | NT | NT | 17/61 (28) | 2/8 | 4/6 | NT | NT | NT | 1/1 | ||||||||
| 68b | 50 | 4/17 | 11/12 | 4/4 | 3/3 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 7/9 | 53/114 (46) | 3/10 | 1/7 | 3/7 | 5/6 | 2/5 | 1/3 | 1/3 | ||||
| 68b | 500 | 14/17 | 11/12 | 73/114 (64) | 5/10 | 2/7 | 6/7 | 1/2 | 3/3 | ||||||||||||||
Includes laboratories with multiple false positives. Detection with an input volume of 50 μl is classified with data for the next 10-fold dilution compared to input with 5 μl. Input with 10 or 15 μl is classified with data for the same dilution compared to input with 5 μl. Other in-house assays include the following, used by one laboratory each: in-house PCR enzyme immunoassay, in-house multiplex PCR gel analysis, and in-house mass spectrometry. RFLP, restriction fragment length polymorphism.
Other commercial assays included the following, used by one laboratory each: Medical Device Microarray, PnE HPV genotyping Luminex, Ampliquality reverse hybridization, Panagene microarray, PANArray HPV, GenoFlow HPV array kit, MolGENTIX multiplex fluorescent PCR, Hybrid Capture 2, Sacace high-risk screen, and Genoserch HPV-31 Luminex.
NT, not tested.