Table 1.
Result scenario for each member of the MTCa
| Multiplex assay | Result with analysis channel and target |
Result interpretationb | ||||
|---|---|---|---|---|---|---|
| Cyan 500 | FAM | HEX | ROX | Cy5 | ||
| 1 | RD 713 | wbbl1 | MTC lepA | RDcanettii1 | IAC MSMEG_0660 | |
| + | + | + | − | + | M. africanum West African 1 | |
| − | + | + | + | + | M. canettii | |
| − | + | + | − | + | M. tuberculosis | |
| − | − | + | − | + | MTC—perform second multiplex | |
| − | − | − | − | + | Not a member of the MTC | |
| − | − | − | − | − | Result invalid, test must be repeated | |
| 2 | M. caprae lepA | lpqT | RD 1 | RD 701 | Cy5 (IAC MSMEG_0660) | |
| + | + | + | − | + | M. caprae | |
| − | + | + | − | + | M. bovis | |
| − | + | − | − | + | M. bovis BCG | |
| − | − | + | + | + | M. africanum West African 2 | |
| − | − | + | − | + | M. pinnipedii | |
| − | − | − | − | + | M. microti | |
| − | − | − | − | − | Result invalid, test must be repeated | |
a
+, positive signal observed; −, positive signal not observed.
b
The interpretations of the results of the multiplex 2 assay take into account the multiplex 1 results.