Table 1.

Primers used by the PCR method in the hot spots of drug target genes

Primera	Nucleotide sequence (5′–3′)	Annealing temp (°C)	Amplicon positionb	Product size (bp)	Source or reference
rpoB-F	TCAAGGAGTTCTTCGGCACC	60.2	761068–761624	557	This study
rpoB-R	CTGCATGTTTGCCCCCAT
katG-F	TGGGCGGACCTGATTGTT	59.5	2459–3051c	593	This study
katG-R	CCGTCCTTGGCGGTGTATT
(mabA-inhA)-F	AAGGCAGAAGCCGAGTAG	57.4	1673100–1673619	520	This study
(mabA-inhA)-R	ACATTCGACGCCAAACAG
gyrA-F	CCGGATCGAACCGGTTGAC	57.2	7340–7766	427	20
gyrA-R	GTTAGGGATGAAATCGACTG
gyrB-F	GTCGTTGTGAACAAGGCTGTG	57.1	6452–6864	413	21
gyrB-R	GTGGAAATATGTTGGCCGTC
rrs-F	GTGAGATGTTGGGTTAAGTCC	55.3	1472913–1473393	481	20
rrs-R	TGGTGCTCCTTAGAAAGGAG

^aF, forward; R, reverse.

^bAccording to the complete sequences of the M. tuberculosis H37Rv genome (accession no. NC_000962), except as noted for katG.

^cAccording to the sequence of the M. tuberculosis H37Rv gene for catalase-peroxidase (accession no. X68081).