Table 3.

Nucleotide and amino acid changes identified in the specific region of six M. tuberculosis drug-resistant associated loci for the corresponding antibiotic

Locus (no. of resistant isolates tested)	Drug	Codon(s)	Changea		No. (%) of isolates	Other mutation
			Nucleotide	Amino acid
rpoBb (77)	RIF	Nonec	None	None	4 (5.2)
		505	TTC→TTT	Phe→Phef	1 (1.3)
		511	CTG→CCG	Leu→Pro	3 (3.9)
		514	TTC→Inse	Phe→Ins	1 (1.3)
		516	GAC→TAC	Asp→Tyr	1 (1.3)
			GAC→GTC	Asp→Val	4 (5.2)
			GAC→TTC	Asp→Phe	1 (1.3)
		526	CAC→AAC	His→Asn	2 (2.6)
			CAC→TAC	His→Tyr	3 (3.9)
			CAC→CTC	His→Leu	2 (2.6)
			CAC→GAC	His→Asp	7 (9.1)
			CAC→CGC	His→Arg	2 (2.6)
			CAC→TGC	His→Cys	2 (2.6)
		529	CGA→AAA	Arg→Lys	1 (1.3)
		531	TCG→TTG	Ser→Leu	29 (37.6)
		511/515d	CTG→CCG	Leu→Pro	1 (1.3)
			ATG→GTG	Met→Val
		511/516	CTG→CCG	Leu→Pro	2 (2.6)
			GAC→GGC	Asp→Gly
		511/516	CTG→CCG	Leu→Pro	1 (1.3)
			GAC→GCC	Asp→Ala
		511/516	CTG→CCG	Leu→Pro	1 (1.3)
			GAC→AAC	Asp→Asn
		513/561	CAA→GAA	Gln→Glu	1 (1.3)
			ATC→GTC	Ile→Val
		516/572	GAC→GGC	Asp→Gly	1 (1.3)
			ATC→CTC	Ile→Leu
		516/572	GAC→TAC	Asp→Tyr	1 (1.3)
			ATC→ATG	Ile→Met*
		531/525	TCG→TTG	Ser→Leu	1 (1.3)
			ACC→ACA	Thr→Thrf
		531/569	TCG→TTG	Ser→Leu	1 (1.3)
			ATC→GTC	Ile→Val*
		531/584	TCG→TTG	Ser→Leu	2 (2.6)
			TTC→TCC	Phe→Ser*
		515/526/535	ATG→GTG	Met→Val	1 (1.3)
			CAC→AAC	His→Asn
			CCC→TCC	Pro→Ser
		531/615/626	TCG→TTG	Ser→Leu	1 (1.3)
			GTG→ATG	Val→Met*
			GAC→GAG	Asp→Glu*
katG (77)	INH	None	None	None	16 (20.8)
		None	None	None	5 (6.5)	–15C→T upstream of mabA
		None	None	None	1 (1.3)	–8T→A upstream of mabA
		191	TGG→CGG	Trp→Arg	1 (1.3)
			TGG→TGA	Trp→Stop*	1 (1.3)
		271	ACT→CCT	Thr→Pro*	2 (2.6)	–15C→T upstream of mabA
		299	GGC→TGC	Gly→Cys	1 (1.3)
			GGC→AGC	Gly→Ser	2 (2.6)
		315	AGC→ACC	Ser→Thr	36 (46.8)
			AGC→ACC	Ser→Thr	2 (2.6)	–15C→T upstream of mabA
			AGC→ACC	Ser→Thr	2 (2.6)	–8T→C upstream of mabA
			AGC→AAC	Ser→Asn	1 (1.3)	–8T→C upstream of mabA
			AGC→ACA	Ser→Thr	3 (3.9)
			AGC→CGC	Ser→Arg	1 (1.3)
			AGC→ATC	Ser→Ile	1 (1.3)
		327	AAA→AAG	Lys→Lysf	1 (1.3)
		328	TGG→TTT	Trp→Phe*	1 (1.3)
mabA-inhA (77)	INH	None	None	None	64 (83.1)
			–8T→A		1 (1.3)
			–8T→C		2 (2.6)	AGC315ACC (Ser→Thr) in katG
			–8T→C		1 (1.3)	AGC315AAC (Ser→Asn) in katG
			–15C→T		5 (6.5)
			–15C→T		2 (2.6)	ATC271CCT (Thr→Pro) in katG
			–15C→T		2 (2.6)	AGC315ACC (Ser→Thr) in katG
gyrA (39)	OFX	None	None	None	1 (2.6)
		21/95	GAG→CAG	Glu→Gln	5 (12.8)
			AGC→ACC	Ser→Thr
		21/95	GAG→CAG	Glu→Gln	1 (2.6)	GAC500CAC (Asp→His) in gyrB
			AGC→ACC	Ser→Thr
		21/95/90	GAG→CAG	Glu→Gln	3 (7.7)
			AGC→ACC	Ser→Thr
			GCG→GTG	Ala→Val
		21/95/91	GAG→CAG	Glu→Gln	2 (5.1)
			AGC→ACC	Ser→Thr
			TCG→CCG	Ser→Pro
		21/95/94	GAG→CAG	Glu→Gln	19 (48.7)
			AGC→ACC	Ser→Thr
			GAC→GGC	Asp→Gly
		21/95/94	GAG→CAG	Glu→Gln	5 (12.8)
			AGC→ACC	Ser→Thr
			GAC→AAC	Asp→Asn
		21/95/94	GAG→CAG	Glu→Gln	1 (2.6)
			AGC→ACC	Ser→Thr
			GAC→GCC	Asp→Ala
		21/95/94	GAG→CAG	Glu→Gln	1 (2.6)
			AGC→ACC	Ser→Thr
			GAC→TAC	Asp→Tyr
		21/95/94	GAG→CAG	Glu→Gln	1 (2.6)	GAA540GAC (Glu→Asp) in gyrB
			AGC→ACC	Ser→Thr
			GAC→GCC	Asp→Ala
gyrB (39)	OFX	None	None	None	37 (94.8)
		500	GAC→CAC	Asp→His	1 (2.6)
		540	GAA→GAC	Glu→Asp	1 (2.6)	GAC94GCC (Asp→Ala) in gyrA
rrs (19)	KAN, CAP	None	None	None	2 (10.5)
	CAP	None	None	None	1 (5.3)
	KAN, AMK, CAP	None	None	None	1 (5.3)
	KAN, AMK		1401A→G		6 (31.6)
	KAN, AMK, CAP		1401A→G		8 (42.1)
	KAN, AMK, CAP		1402C→T		1 (5.3)

^a*, Mutations not previously reported.

^bThe amino acid numbering is based on homologous mutations in E. coli.

^cNone, no mutation.

^dMultiple mutations in one isolate.

^eIns, insertion.

^fSilent mutation.