Figure 1. Mutations in AtMBF1 genes enhance seed dormancy. Seeds from WT, abc-, and abc- +c plants were surface-sterilized and stratified or not at 4°C for 2 d in the dark to break dormancy. Seeds were plated on ATS medium with 0.8% agar and placed on a growth chamber at 23°C with a 16 h-light photoperiod. (A) The percentage of germination was scored after 24 h. (B) The percentage of 0.7 stage according to Boyes et al.¹⁹ was evaluated after 48 h. Approximately, 100 seeds were processed per line in each experiment. Data are mean values (± SE) of five independent experiments. Different letters indicate a significant difference at p < 0.05 (Tukey's test).