Figure 3.

Low-power (a) and high-power (b) micrographs of flat mounted, post-natal day 3 mouse retina. (a) Dual-staining with isolectin-B4 (green) and an anti-fibronectin antibody (blue) reveals leading edge endothelial tip cells (white arrows) migrating towards the retinal periphery (rp) guided by underlying fibronectin matrix (arrowheads). Numerous microglial cells, also stained with isolectin-B4, are conspicuous in the avascular region of the retina (yellow arrows). (b) Dual immunolabelling of the leading edge of the vascular plexus with isolectin-B4 (red) and transcardially administered FITC–dextran (green) reveals perfused retinal vascular plexus (arrowheads) segments immediately behind endothelial tip cells (arrows).