Figure 10. Autophagic response in large human hepatocytes during hypoxia and H-R. The top panel demonstrates a representative flow cytometry plot to illustrate the levels of MDC staining of PV/large human hepatocytes during normoxia (blue), hypoxia (hatched red) and H-R (solid gray). The plot on the left hand side of the flow cytometric plot represents a typical FS vs. SS plot, similar to that shown in Figure 1A. The FS vs. SS plot is from a H-R sample but similar plots were obtained during normoxia and hypoxia (data not shown). The gating protocol applied to human hepatocytes to analyze PV/large human hepatocytes is shown on the FS vs. SS plot. The area of interest of each flow cytometry plot is marked by a vertical ellipse. The bottom panel illustrates the effects of 3-MA pre-treatment upon PV/large human hepatocyte autophagy and apoptosis during H-R. The effects of H-R alone are shown in blue and the effects of 3-MA pretreatment are shown in solid gray. The bar chart shows composite data from four separate experiments. Data are expressed as mean ± SE (*p < 0.05 relative to basal, Mann-Whitney test).