Figure 1. MB treatment reduces tau forms in organotypic slice cultures. Slice cultures from JNPL3 mice (n = 6 mice per condition, three mice shown) were treated with either DMSO vehicle (C, control) or 0.02 μM MB (T, treated). (A) shows the results of immunoblotting with an antibody recognizing human tau (CP27) on the total and sarkosyl insoluble, aggregated tau fractions. The total protein fraction was also assayed for levels of tau phosphorylated at epitopes ser199, ser262, ser422, and ser396/404. Images were quantified and the chemiluminescence signal from MB treated hemibrain slices was expressed as percentage of signal from control treated hemibrain from the same animal (signal ± SE) (B). Total tau levels were unchanged with MB treatment. MB significantly reduced (p < 0.05) the level of sarkosyl insoluble, aggregated tau. Significant reduction was seen in tau hyperphosphorylated at ser199, ser262, ser422 and ser396/404 (phospho-tau epitope normalized to total tau). *p < 0.05.