Figure 2. MB treatment modulates autophagy markers in organotypic slice cultures. Samples utilized in Figure 1 were assayed for levels of autophagy markers. (A) shows the results of immunoblotting with antibodies against p62, cathepsin D (catD), BECN1 and LC3. Tubulin (tub) is shown as loading control. (B) shows the results quantified. 0.02 μM MB produced a significant decrease in p62 levels and an increase in catD, BECN1 and LC3-II signal relative to control treated slices **p < 0.01.