Figure 4. Tau colocalizes with AVs. Primary neuronal cultures from GFP-LC3 mice were incubated with aggregated human tau for 18 h prior to fixation and labeling with fluorescently tagged antibody CP27 (human tau). (A) shows a confocal slice at 0.3 μm planar resolution indicating the green immunofluorescence signal from GFP-LC3; (B) indicates the red immunofluorescence signal from human tau aggregates. (C) shows the merged images (yellow) indicating that tau can colocalize with autophagic vacuoles, illustrated in the enlarged inset shown in (D). Human tau in AVs is indicated by the arrow.