Figure 6. MB induces autophagy in CHO cells. Tau-transfected CHO cells were incubated for 6 h with vehicle or 0.01 μM MB (n = 6 per treatment). (A) shows immunoblots probed with antibodies against total tau, p62, BECN1 and LC3. (B) shows quantification of the immunoblots. Cells treated with MB (gray bars) had significantly reduced tau levels compared with vehicle-treated (black bars) cells, while the levels of BECN1 and LC3-II were increased. No effect on p62 levels was seen at this time point. All bars represent average ± SE, *p < 0.05.