Figure 7.

Kinase phosphorylation is altered with MB treatment. CHO cells treated with MB or rapamycin, a known autophagy inducer, were immunoblotted for kinase phosphorylation to assess activation status. To test whether MB acted in a similar way to rapamycin, cells were incubated with vehicle, rapamycin or MB for 6 h. (A) shows immunoblots for antibodies listed. (B) shows the quantification. The ratio between phosphorylated kinase at a relevant epitope and total kinase was determined in control (black bars), rapamycin (gray bars) and MB (hashed bars). MB significantly reduced levels of phospho-mTOR ser2448. Rapamycin also reduced levels of phospho-mTOR ser2448. Both rapamycin and MB reduced levels of phospho-p70 thr389 and phospho-IRS ser302. Rapamycin and MB also produced a significant increase in phospho-Akt ser473 and phospho-GSK-3β ser9. All bars represent average values and error bars SEM, *p < 0.05.