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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Ann Neurol. 2011 Dec;70(6):920–931. doi: 10.1002/ana.22549

Figure 8.

Figure 8

Gleevec and PDGF-AA neutralizing antibody reduced Evans blue extravasation 24 hours following thrombin injection in mice. PDGFR-α antagonist, Gleevec (60 mg/kg) was administered 1 hour following thrombin (5 U) injection. Inactive PDGF-AA antibody (PDGF-AA Ab) or PDGF-AA antibody (PDGF-AA Ab, 1.2 μg) was co-injected with thrombin (5 U) into right basal ganglia. (A) Evans blue extravasation in the ipsilateral hemisphere 24 hours following operation in sham, thrombin (5 U) and Gl treatment (60 mg/kg) groups; (B) Immunoprecipitation assay (IP) for phosphor-PDGFR-α level with phosphotyrosine-specific antibody (P-tyr) in the ipsilateral hemisphere 6 hours following thrombin injection in sham, thrombin (5 U) and Gl treatment (60 mg/kg) mice. The precipitated protein was also visualized with PDGFR-α-specific antibodies (R-alpha). IgG was visualized as a loading control. (D) Western blot assay for PDGF-AA in Sham, ipsilateral (Ipsi) and contralateral (Contra) hemisphere in thrombin injection mice 6 hours following operation; (F) Evans blue extravasation in the ipsilateral hemisphere 24 hours following operation in thrombin (5 U), thrombin (5 U)+inactive PDGF-AA antibody (PDGF-AA Ab), and PDGF-AA antibody (PDGF-AA Ab, 1.2 μg) mice. Quantification of B and D is shown in C and E, respectively. n = 5–8 mice per group. Error bars represent mean ± standard error of the mean. # p < 0.05 vs Sham; * p < 0.05 vs Thrombin; @ p < 0.05 vs Contra; & p < 0.05 vs Thrombin+inactive Ab.