Flow cytometric analysis of in vivo attachment of α-DC-ZZ-BNC complexes to splenic DCs. (A) Distributions of Cy5-derived fluorescence in splenic DCs isolated from Cy5-labeled α-DC-ZZ-BNC complex-injected mice and untreated mice are indicated by closed and open histograms, respectively. (B) Distributions of Cy5-derived fluorescence in splenic CD11c− cells isolated from Cy5-labeled α-DC-ZZ-BNC complex-injected mice.
Notes: The percentages (%) of ZZ-BNC+ cells in DCs and CD11c− cells are indicated as numbers.
Abbreviations: DC, dendritic cell; ZZ-BNC, BNC displaying ZZ domains.