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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Cell Host Microbe. 2012 Jul 19;12(1):86–96. doi: 10.1016/j.chom.2012.05.012

Fig. 1. Identification of cell surface protein alterations in HCMV-infected cells.

Fig. 1

Fibroblasts were mock-infected or infected at a multiplicity of 5 IU/cell.

(A) Specificity of biotin labeling: Avidin-purified cell surface protein preparations were assayed by Western blot for β-actin, insulin receptor (IR), MHC-class I receptor (HLA-B/C), and prostaglandin receptor 2 (PGE2R2).

(B) Purification of biotinylated proteins: Avidin-purified samples (~50 µg protein in +biotin samples) were subjected to electrophoresis and Coomassie stained.

(C) Reproducibility of MS data across independent experiments: Normalized spectral counts of infected cell proteins isolated at 6 (■), 24 (●) and 72 hpi (▲) were plotted from two independent experiments. Dotted-lines mark a 2-fold difference in counts.